Fluorescent reporter signals, EGFP and DsRed, encoded in HIV-1 facilitate the detection of productively infected cells and cell-associated viral replication levels

Fluorescent reporter signals, EGFP and DsRed, encoded in HIV-1 facilitate the detection of productively infected cells and cell-associated viral replication levels

Flow cytometric analysis is a reliable and convenient method for investigating molecules at the single cell level. Previously, recombinant human immunodeficiency virus type 1 (HIV-1) strains were constructed that express a fluorescent reporter, either enhanced green fluorescent protein or DsRed, whi...

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Bibliographic Details
Main Authors: Kazutaka eTerahara, Takuya eYamamoto, Yu-ya eMitsuki, Kentaro eShibusawa, Masayuki eIshige, Fuminori eMizukoshi, Kazuo eKobayashi, Yasuko eTsunetsugu-Yokota
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-01-01
Series:Frontiers in Microbiology
Subjects:
Flow Cytometry
HIV-1
Gag
DsRed
EGFP
productive infection
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00280/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2011.00280/full

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