Solid-phase extraction for determination of caffeine and its dimethylxanthine metabolites in rat urine using high performance liquid chromatography and its application

A solid-phase extraction (SPE) method for use with high performance liquid chromatography to measure caffeine (CF) and its metabolites in rat urine was developed and validated. Oasis HLB cartridges were used and washed with water, 1% acetic acid: 3% methanol-water (0.5:99.5 v/v), and 1% ammonium h...

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Bibliographic Details
Main Authors: Keson Trakunram, Benjamas Janchawee, Pritsana Raungrut, Sathaporn Prutipanlai
Format: Article
Language:English
Published: Prince of Songkla University 2018-08-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
SPE
rat
Online Access:https://rdo.psu.ac.th/sjstweb/journal/40-4/5.pdf