Extraction and sequencing of single nuclei from murine skeletal muscles

Extraction and sequencing of single nuclei from murine skeletal muscles

Summary: Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows us to...

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Bibliographic Details
Main Authors: Matthieu Dos Santos, Stamatia Gioftsidi, Stéphanie Backer, Léo Machado, Frederic Relaix, Pascal Maire, Philippos Mourikis
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
RNAseq
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004019

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http://www.sciencedirect.com/science/article/pii/S2666166721004019

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