Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology

Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology

MPT64 is a specific protein that is secreted by Mycobacterium tuberculosis complex (MTBC). The objective of this study was to obtain optimum culture conditions for MPT64 synthetic gene expression in Escherichia coli BL21 (DE3) by response surface methodology (RSM). The RSM was undertaken to optimize...

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Bibliographic Details
Main Authors: Sri Agung Fitri Kusuma, Ida Parwati, Tina Rostinawati, Muhammad Yusuf, Muhammad Fadhlillah, Risa R. Ahyudanari, Yaya Rukayadi, Toto Subroto
Format: Article
Language:English
Published: Elsevier 2019-11-01
Series:Heliyon
Subjects:
Environmental science
Microbiology
MPT64
Response surface methodology
Box-Behnken design
Rhamnose
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844019364011

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http://www.sciencedirect.com/science/article/pii/S2405844019364011

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