Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats
Abstract.: We investigated whether increased contractile responsiveness to epidermal growth factor (EGF) is associated with altered activation of mitogen-activated protein kinase (MAPK) in the aortic smooth muscle of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. EGF induced contraction...
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Elsevier
2006-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319344585 |
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doaj-0069a0299d6d4f36a9aa42e8e704cbf42020-11-25T01:26:21ZengElsevierJournal of Pharmacological Sciences1347-86132006-01-011012135143Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive RatsKim Junghwan0Lee Chang-Kwon1Park Hyo-Jun2Kim Hyo Jin3So Hyun Ha4Lee Keun Sang5Lee Hwan Myung6Roh Hui Yul7Choi Wahn Soo8Park Tae Kyu9Kim Bokyung10Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Immunology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Biotechnology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, KoreaDepartment of Physiology, College of Medicine, Institute of Biomedical Science and Technology, College of Biomedical & Health Science, Konkuk University, Danwol-dong 322, Choongju 380-701, Korea; Corresponding author. bkkim2@kku.ac.krAbstract.: We investigated whether increased contractile responsiveness to epidermal growth factor (EGF) is associated with altered activation of mitogen-activated protein kinase (MAPK) in the aortic smooth muscle of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. EGF induced contraction and MAPK activity in aortic smooth muscle strips, which were significantly increased in tissues from the DOCA-salt hypertensive rats compared with those from sham-operated rats. AG1478, PD98059, and LY294002, inhibitors of EGF receptor (EGFR) tyrosine kinase, MAPK/extracellular signal-regulated kinase (ERK) kinase, and phosphatidylinositol 3-kinase (PI3K), respectively, inhibited the contraction and the activity of ERK1/2 that were elevated by EGF. Y27632 and GF109203X, inhibitors of Rho kinase and protein kinase C, respectively, attenuated EGF-induced contraction, with no diminution of ERK1/2 activity. Although EGF also elevated the activity of EGFR tyrosine kinase in both sham-operated and DOCA-salt hypertensive rats, the expression and the magnitude of activation did not differ between strips. These results strongly suggest that EGF induces contraction by the activation of ERK1/2, which is regulated by the PI3K pathway in the aortic smooth muscle of DOCA-salt hypertensive rats. Keywords:: epidermal growth factor, hypertension, vasoconstriction, mitogen-activated protein kinase, phosphatidylinositol 3-kinasehttp://www.sciencedirect.com/science/article/pii/S1347861319344585 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Kim Junghwan Lee Chang-Kwon Park Hyo-Jun Kim Hyo Jin So Hyun Ha Lee Keun Sang Lee Hwan Myung Roh Hui Yul Choi Wahn Soo Park Tae Kyu Kim Bokyung |
| spellingShingle |
Kim Junghwan Lee Chang-Kwon Park Hyo-Jun Kim Hyo Jin So Hyun Ha Lee Keun Sang Lee Hwan Myung Roh Hui Yul Choi Wahn Soo Park Tae Kyu Kim Bokyung Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats Journal of Pharmacological Sciences |
| author_facet |
Kim Junghwan Lee Chang-Kwon Park Hyo-Jun Kim Hyo Jin So Hyun Ha Lee Keun Sang Lee Hwan Myung Roh Hui Yul Choi Wahn Soo Park Tae Kyu Kim Bokyung |
| author_sort |
Kim Junghwan |
| title |
Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats |
| title_short |
Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats |
| title_full |
Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats |
| title_fullStr |
Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats |
| title_full_unstemmed |
Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats |
| title_sort |
epidermal growth factor induces vasoconstriction through the phosphatidylinositol 3-kinase-mediated mitogen-activated protein kinase pathway in hypertensive rats |
| publisher |
Elsevier |
| series |
Journal of Pharmacological Sciences |
| issn |
1347-8613 |
| publishDate |
2006-01-01 |
| description |
Abstract.: We investigated whether increased contractile responsiveness to epidermal growth factor (EGF) is associated with altered activation of mitogen-activated protein kinase (MAPK) in the aortic smooth muscle of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. EGF induced contraction and MAPK activity in aortic smooth muscle strips, which were significantly increased in tissues from the DOCA-salt hypertensive rats compared with those from sham-operated rats. AG1478, PD98059, and LY294002, inhibitors of EGF receptor (EGFR) tyrosine kinase, MAPK/extracellular signal-regulated kinase (ERK) kinase, and phosphatidylinositol 3-kinase (PI3K), respectively, inhibited the contraction and the activity of ERK1/2 that were elevated by EGF. Y27632 and GF109203X, inhibitors of Rho kinase and protein kinase C, respectively, attenuated EGF-induced contraction, with no diminution of ERK1/2 activity. Although EGF also elevated the activity of EGFR tyrosine kinase in both sham-operated and DOCA-salt hypertensive rats, the expression and the magnitude of activation did not differ between strips. These results strongly suggest that EGF induces contraction by the activation of ERK1/2, which is regulated by the PI3K pathway in the aortic smooth muscle of DOCA-salt hypertensive rats. Keywords:: epidermal growth factor, hypertension, vasoconstriction, mitogen-activated protein kinase, phosphatidylinositol 3-kinase |
| url |
http://www.sciencedirect.com/science/article/pii/S1347861319344585 |
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