Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay

Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay

Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated in BHK-21 cell line in our laboratory. The Nuc...

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Bibliographic Details
Main Authors:	Gaya Prasad, Pawan Kumar, Supriya A, P Minakshi, Ranjan Koushlesh, T.M. Vishwaradhya
Format:	Article
Language:	English
Published:	Veterinary World 2013-06-01
Series:	Veterinary World
Subjects:	Bluetongue BTV21 RT-PCR Real time RT-PCR ns1 gene
Online Access:	http://www.veterinaryworld.org/Vol.6/August - 2013/Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay.pdf

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