Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay
Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated in BHK-21 cell line in our laboratory. The Nuc...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Veterinary World
2013-06-01
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Series: | Veterinary World |
Subjects: | |
Online Access: | http://www.veterinaryworld.org/Vol.6/August - 2013/Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay.pdf |