Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease.

by Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya
Published 2017-01-01

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

by Tetsushi Sakuma, Mitsumasa Takenaga, Yoshinori Kawabe, Takahiro Nakamura, Masamichi Kamihira, Takashi Yamamoto
Published 2015-10-01

Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference.

by Yusuke Sato'o, Junzo Hisatsune, Liansheng Yu, Tetsushi Sakuma, Takashi Yamamoto, Motoyuki Sugai
Published 2018-01-01

PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells

by Yusuke Kaku, Atsuhiro Taguchi, Shunsuke Tanigawa, Fahim Haque, Tetsushi Sakuma, Takashi Yamamoto, Ryuichi Nishinakamura
Published 2017-07-01

A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector.

by Hirotaka Ebina, Yuka Kanemura, Naoko Misawa, Tetsushi Sakuma, Tomoko Kobayashi, Takashi Yamamoto, Yoshio Koyanagi
Published 2015-01-01

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

by Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Published 2017-05-01

MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner

by Wenyu Miao, Katsuya Sakai, Ryu Imamura, Kenichiro Ito, Hiroaki Suga, Tetsushi Sakuma, Takashi Yamamoto, Kunio Matsumoto
Published 2018-10-01

Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors

by Shunsuke Tanigawa, Hidekazu Naganuma, Yusuke Kaku, Takumi Era, Tetsushi Sakuma, Takashi Yamamoto, Atsuhiro Taguchi, Ryuichi Nishinakamura
Published 2019-08-01

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

by Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Noriyuki Yanaka, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Published 2016-08-01

Biased genome editing using the local accumulation of DSB repair molecules system

by Shota Nakade, Keiji Mochida, Atsushi Kunii, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, Tetsushi Sakuma, Takashi Yamamoto
Published 2018-08-01

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

by Takuya Sato, Tetsushi Sakuma, Tetsuhiro Yokonishi, Kumiko Katagiri, Satoshi Kamimura, Narumi Ogonuki, Atsuo Ogura, Takashi Yamamoto, Takehiko Ogawa
Published 2015-07-01

The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation

by Tatsuo Miyamoto, Kosuke Hosoba, Hiroshi Ochiai, Ekaterina Royba, Hideki Izumi, Tetsushi Sakuma, Takashi Yamamoto, Brian David Dynlacht, Shinya Matsuura
Published 2015-02-01

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

by Ken-ichi T. Suzuki, Yukiko Isoyama, Keiko Kashiwagi, Tetsushi Sakuma, Hiroshi Ochiai, Naoaki Sakamoto, Nobuaki Furuno, Akihiko Kashiwagi, Takashi Yamamoto
Published 2013-03-01

Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene

by Yasuyoshi Tanaka, Takefumi Sone, Norimichi Higurashi, Tetsushi Sakuma, Sadafumi Suzuki, Mitsuru Ishikawa, Takashi Yamamoto, Jun Mitsui, Hitomi Tsuji, Hideyuki Okano, Shinichi Hirose
Published 2018-04-01

Quantitative assay for TALEN activity at endogenous genomic loci

by Yu Hisano, Satoshi Ota, Kazuharu Arakawa, Michiko Muraki, Nobuaki Kono, Kazuki Oshita, Tetsushi Sakuma, Masaru Tomita, Takashi Yamamoto, Yasushi Okada, Atsuo Kawahara
Published 2013-02-01

Microhomology-assisted scarless genome editing in human iPSCs

by Shin-Il Kim, Tomoko Matsumoto, Harunobu Kagawa, Michiko Nakamura, Ryoko Hirohata, Ayano Ueno, Maki Ohishi, Tetsushi Sakuma, Tomoyoshi Soga, Takashi Yamamoto, Knut Woltjen
Published 2018-03-01

EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD

by Ginto George, Satoshi Ninagawa, Hirokazu Yagi, Taiki Saito, Tokiro Ishikawa, Tetsushi Sakuma, Takashi Yamamoto, Koshi Imami, Yasushi Ishihama, Koichi Kato, Tetsuya Okada, Kazutoshi Mori
Published 2020-02-01

The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos.

by Kei Miyamoto, Ken-Ichi T Suzuki, Miyuki Suzuki, Yuto Sakane, Tetsushi Sakuma, Sarah Herberg, Angela Simeone, David Simpson, Jerome Jullien, Takashi Yamamoto, J B Gurdon
Published 2015-01-01

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9

by Hongmei Lisa Li, Naoko Fujimoto, Noriko Sasakawa, Saya Shirai, Tokiko Ohkame, Tetsushi Sakuma, Michihiro Tanaka, Naoki Amano, Akira Watanabe, Hidetoshi Sakurai, Takashi Yamamoto, Shinya Yamanaka, Akitsu Hotta
Published 2015-01-01

Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes

by Shunsuke Tanigawa, Mazharul Islam, Sazia Sharmin, Hidekazu Naganuma, Yasuhiro Yoshimura, Fahim Haque, Takumi Era, Hitoshi Nakazato, Koichi Nakanishi, Tetsushi Sakuma, Takashi Yamamoto, Hidetake Kurihara, Atsuhiro Taguchi, Ryuichi Nishinakamura
Published 2018-09-01