Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay

by Marie Wunsch, Richard Caspell, Stefanie Kuerten, Paul V. Lehmann, Srividya Sundararaman
Published 2015-01-01

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells

by Jodi Hanson, Srividya Sundararaman, Richard Caspell, Edith Karacsony, Alexey Y. Karulin, Paul V. Lehmann
Published 2015-01-01

A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool

by Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten, Paul V. Lehmann
Published 2017-12-01

ELISPOTs Produced by CD8 and CD4 Cells Follow Log Normal Size Distribution Permitting Objective Counting

by Alexey Y. Karulin, Kinga Karacsony, Wenji Zhang, Oleg S. Targoni, Ioana Moldovan, Marcus Dittrich, Srividya Sundararaman, Paul V. Lehmann
Published 2015-01-01

Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity

by Marie Wunsch, Wenji Zhang, Jodi Hanson, Richard Caspell, Alexey Y. Karulin, Mascha S. Recks, Stefanie Kuerten, Srividya Sundararaman, Paul V. Lehmann
Published 2015-08-01

High-Throughput GLP-Capable Target Cell Visualization Assay for Measuring Cell-Mediated Cytotoxicity

by Anna Welter, Srividya Sundararaman, Ruliang Li, Ting Zhang, Alexey Y. Karulin, Alexander Lehmann, Villian Naeem, Diana R. Roen, Stefanie Kuerten, Paul V. Lehmann
Published 2018-04-01

High Reproducibility of ELISPOT Counts from Nine Different Laboratories

by Srividya Sundararaman, Alexey Y. Karulin, Tameem Ansari, Nadine BenHamouda, Judith Gottwein, Sreenivas Laxmanan, Steven M. Levine, John T. Loffredo, Stephanie McArdle, Christine Neudoerfl, Diana Roen, Karina Silina, Mackenzie Welch, Paul V. Lehmann
Published 2015-01-01