Optimized expression and purification for high-activity preparations of algal [FeFe]-hydrogenase.

by Iftach Yacoby, Lotta Tollstoy Tegler, Sergii Pochekailov, Shuguang Zhang, Paul W King
Published 2012-01-01

Site saturation mutagenesis demonstrates a central role for cysteine 298 as proton donor to the catalytic site in CaHydA [FeFe]-hydrogenase.

by Simone Morra, Alberto Giraudo, Giovanna Di Nardo, Paul W King, Gianfranco Gilardi, Francesca Valetti
Published 2012-01-01

The Physiological Functions and Structural Determinants of Catalytic Bias in the [FeFe]-Hydrogenases CpI and CpII of Clostridium pasteurianum Strain W5

by Jesse B. Therien, Jacob H. Artz, Saroj Poudel, Trinity L. Hamilton, Zhenfeng Liu, Seth M. Noone, Michael W. W. Adams, Paul W. King, Donald A. Bryant, Donald A. Bryant, Eric S. Boyd, John W. Peters
Published 2017-07-01