Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity.

by Megan Basila, Melissa L Kelley, Anja van Brabant Smith
Published 2017-01-01

Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition.

by Emily M Anderson, Shawn McClelland, Elena Maksimova, Žaklina Strezoska, Megan Basila, Alexandra E Briner, Rodolphe Barrangou, Anja van Brabant Smith
Published 2018-01-01