Systematic Functional Analysis of Active-Site Residues in l‑Threonine Dehydrogenase from Thermoplasma volcanium

by Morgan Desjardins, Wai Shun Mak, Terrence E. O’Brien, Dylan Alexander Carlin, Dean J. Tantillo, Justin B. Siegel
Published 2017-07-01

Thermal stability and kinetic constants for 129 variants of a family 1 glycoside hydrolase reveal that enzyme activity and stability can be separately designed.

by Dylan Alexander Carlin, Siena Hapig-Ward, Bill Wayne Chan, Natalie Damrau, Mary Riley, Ryan W Caster, Bowen Bethards, Justin B Siegel
Published 2017-01-01

Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables the Discovery of Structural Features Correlated with Kinetic Constants.

by Dylan Alexander Carlin, Ryan W Caster, Xiaokang Wang, Stephanie A Betzenderfer, Claire X Chen, Veasna M Duong, Carolina V Ryklansky, Alp Alpekin, Nathan Beaumont, Harshul Kapoor, Nicole Kim, Hosna Mohabbot, Boyu Pang, Rachel Teel, Lillian Whithaus, Ilias Tagkopoulos, Justin B Siegel
Published 2016-01-01