Article Biomarkers, Tumor Breast Neoplasms Case-Control Studies Cell-Free Nucleic Acids DNA determination DNA marker Estrogens Female Fluorescence Humans Middle Aged Neoplasm Staging PUM1 gene PUM1 protein, human RNA binding protein RNA-Binding Proteins RNase P gene ROC Curve Ribonuclease P Sensitivity and Specificity Triple Negative Breast Neoplasms adult advanced cancer blood breast cancer breast tumor cancer prognosis cancer staging case control study