A one pot, one step, precision cloning method with high throughput capability.

by Carola Engler, Romy Kandzia, Sylvestre Marillonnet
Published 2008-01-01

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.

by Carola Engler, Ramona Gruetzner, Romy Kandzia, Sylvestre Marillonnet
Published 2009-01-01

Quick and clean cloning: a ligation-independent cloning strategy for selective cloning of specific PCR products from non-specific mixes.

by Frank Thieme, Carola Engler, Romy Kandzia, Sylvestre Marillonnet
Published 2011-01-01

Assembly of designer TAL effectors by Golden Gate cloning.

by Ernst Weber, Ramona Gruetzner, Stefan Werner, Carola Engler, Sylvestre Marillonnet
Published 2011-01-01

A modular cloning system for standardized assembly of multigene constructs.

by Ernst Weber, Carola Engler, Ramona Gruetzner, Stefan Werner, Sylvestre Marillonnet
Published 2011-02-01