Article CRISPR Cas system CRISPR-CAS9 system CRISPR-Cas Systems CRISPR-Cas9 DNA extraction DNA sequence Gene Editing Humans Loss of Function Mutation Pluripotent Stem Cells RNA extraction RNA sequence RNA, Guide Sanger sequencing Streptococcus pyogenes bioinformatics cell cloning cell differentiation cell viability clonal derivation colony formation controlled study copy number variation frameshift mutation gene amplification gene editing gene expression gene function gene mutation