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Gibson assembly
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Inteins
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141“... genetic elements transferred from phylogenetically distant organisms, putative inteins were identified...”
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142by Mistry, Ishna“... split Nostoc punctiforme DnaE inteins, onto the chromosome of a mammalian cell line, to allow inducible...”
Published 2016
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143by Andrzej Stanisław Cieplak, Andrzej Stanisław Cieplak, Andrzej Stanisław Cieplak“..., in analogy to folding of split inteins. The free energy of binding is minimized when the antiparallel...”
Published 2019-05-01
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144by Martinez, Sarah Jane“... of the two domains of RSU using intein technology. Intein technology provides a novel means by which isotope...”
Published 2004
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146by Al-Ali, Hassan“... split inteins of the gene dnaE from Nostoc punctiforme [(N)NpuDnaE] and Synechocystis sp. strain PCC6803...”
Published 2010
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147by Lohmueller, Jason Jakob“... and AND and NAND logic gates by reconstituting split zinc finger activators and repressors with split inteins. Next...”
Published 2013
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148by Bürglin Thomas R“... domain proteins, the inteins and VWA-Vint proteins represent three different families of Hint domain...”
Published 2008-03-01
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149by Kim Brügger, Lanming Chen, Markus Stark, Arne Zibat, Peter Redder, Andreas Ruepp, Mariana Awayez, Qunxin She, Roger A. Garrett, Hans-Peter Klenk“.... The genome carries no detectable transposable or integrated elements, no inteins, and introns are exclusive...”
Published 2007-01-01
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150by Huettinger, Karl“... and AGT or intein fusion domains (necessary for labeling) for eukaryotic and prokaryotic expression were...”
Published 2010
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151by Alireza G. Senejani, J. Peter Gogarten“... or intein containing genes. They lead to the rapid spread of the genetic element that hosts them by a...”
Published 2007-01-01
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153“... intein, Ssp DanB, New England Biolabs) was introduced between GST (glutathione-S-transferase) and EGFP...”
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154by Joachim Zettler, Simone Eppmann, Alena Busche, Dina Dikovskaya, Volker Dötsch, Henning D Mootz, Tim Sonntag“...Split intein enabled protein trans-splicing (PTS) is a powerful method for the ligation of two...”
Published 2013-01-01
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155“... of recombinant proteins using nattokinase as an example. The fusion partners consisting of intein-oleosin...”
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156“... intein to the N-terminus of a target protein allowed protein purification with a pH and temperature shift...”
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157by Xu-Jing Wang, Xi Jin, Bao-Qing Dun, Ning Kong, Shi-Rong Jia, Qiao-Ling Tang, Zhi-Xing Wang“... to the scientists and public. This work aimed to assess the effect of intein-mediated gene splitting on containment...”
Published 2014-01-01
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158“... proteins, the thioredoxin-, CBD-intein-, and MalE- fused proteins were constructed. Soluable form fusion...”
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159“... or ELK16) and an intein molecule. In such a scheme, a target protein is first expressed as active protein...”
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160“... with intein (INT) and target protein, i.e., enchanced green fiuorescence protein (EGFP), were fused together...”
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