genome editing CRISPR Cas system 5 gene editing 5 genetics 5 Article 4 CRISPR 3 CRISPR-Cas Systems 3 Gene Editing 3 clustered regularly interspaced short palindromic repeat 3 guide RNA 3 human 3 nonhuman 3 wheat 3 CRISPR-CAS9 system 2 Cas9 2 Clustered Regularly Interspaced Short Palindromic Repeats 2 DNA sequence 2 Plant Breeding 2 RNA sequence 2 RNA, Guide 2 Sanger sequencing 2 Triticum 2 apolipoprotein B 2 cholesterol 2 controlled study 2 functionality 2 gene knockout 2 gene sequence 2 hexaploidy 2 plant breeding 2