Article 1 Gibson assembly 1 Inteins 1 Mycobacterium smegmatis 1 Protein Splicing 1 Proteins 1 RNA Splicing 1 RNA splicing 1 agar 1 bacterial cell 1 conditional protein splicing genetics 1 in vitro study 1 intein 1 inteins 1 kanamycin 1 molecular cloning 1 mycobacteria 1 nonhuman 1 phenotype 1 priority journal 1 protein 1 protein analysis 1 protein assembly 1 protein splicing 1 quantitative analysis 1 spot titer 1 translation regulation 1