Article 1 Escherichia coli 1 Keio collection Knockout 1 Polar effects 1 Proteomics 1 Single gene deletion 1 bacterial gene 1 bacterium mutant 1 binding site 1 controlled study 1 deletion mutant 1 down regulation 1 gene deletion 1 gene location 1 genetic transcription 1 molecular library 1 nonhuman 1 operon 1 proteomics 1 ribosome 1 transcriptome sequencing 1 translation initiation 1 upregulation 1