Amino Acid Sequence 1 Animals 1 Antigenicity 1 Antigens, Bacterial 1 Article 1 BCG vaccine 1 Bacterial Proteins 1 CFP-10 protein (71-85), Mycobacterium tuberculosis 1 Cattle 1 Cloning, Molecular 1 Codon 1 Codon optimization ESAT-6 protein, Mycobacterium tuberculosis 1 Escherichia coli 1 Fusion protein 1 Gene Expression 1 Genetic Vectors 1 His-His-His-His-His-His 1 Histidine 1 Immunogenicity, Vaccine 1 Interferon-gamma 1 Mycobacterium bovis 1 Mycobacterium tuberculosis 1 Mycobacterium tuberculosis complex 1 Oligopeptides 1 Peptide Fragments 1 Recombinant Fusion Proteins 1 Sequence Alignment 1 Soluble ESAT6-CFP10 1 Synthetic gene 1