Effect of DNase treatment on RNA extraction from preimplantation murine embryos

Effect of DNase treatment on RNA extraction from preimplantation murine embryos

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The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation e...

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Bibliographic Details
Main Authors: Abdullah, B (Author), Hoh, BP (Author), Kadir, SHSA (Author), Mohd-Fazirul, M (Author), Nor-Ashikin, MNK (Author), Norhazlin, J (Author), Norita, S (Author), Rajikin, MH (Author), Razif, D (Author), Wan-Hafizah, WJ (Author)
Format: Article
Language:English
Published: 2015
Subjects:
Bioanalyzer
DNase treatment
Microarray
Preimplantation murine embryos
QUANTIFICATION
RNA extraction
RT-PCR
RT-qPCR
Online Access:View Fulltext in Publisher

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