| Summary: | Muntingia calabUra t.,Muntingiaceae, is a medicinal plant-for-various pain-related diseases. The aims of the' present study were to detennine the antitiociceptive profile and to.elucidate the possible mech, anisMs of antinociception of petroleum ether partition obtained from crude- Methanol extract of M.. Satabura leaves using various animal models. The antinociceptive profile of petroleum ether fraction (given orak 100, 250 and 500 mgikawaS established using the in vivo chemicals (acetic acid-induced abdominal constriction and formalin-induced paW licking:test) and thermal (hot,plate test) models of bociception. The role of glutamate, TRPV1 receptor, bradykinin, protein kinase C,. potassiuin channels, and various opioid and non-opioid receptors in modulating the partition's antinociceptie activity was also determined. The results obtained demonstrated that.pettoleum ether.,partitionexerted significant (p < 0.05) antinociception in all the chemicalS-, thermal-, capsaicin-, glutamate-, bradykinin, and 'phorbol 12-myristate 13-acetate (PMA)-induced nociception models. The antinocicebtive activity was reversed following pretreatment with opioid, antagonists (i.e. naloxone, (3-funaltrexamine, naltrindole and nor-binaltorphimine), and-the non-opioid receptor antagonistt (i.e. Pindolot (a 13-adrenoceptor), halciperidol (a mpn-selective dopartinergic), atropine (a non-selective cholinergic-receptor), caffeine (a non-selective adenosinergic receptor), and yohimbine (an 62-noradrenergic)): In addition, pretreatment 'with L-arginine (a-nitric oxide (NO)-donor), NG-nitro-L-arginine methyl esters (i-NAME; an inhibitor of NO synthase,(NOS)), Methylene blue (MB; an inhibitor df cyclic-guanosine monophoSphate-(c,GMP) pathway), or theft- combination failed to inhibit petroleum ether partition's antingeiception. In conclusion, petroleum ethet.partition exerts.antinociceptive activity-at,the peripheral and central levels via the modulation of; partly, the opioid K and 8) and Several non-opioids (i.e. (3-adrenergic, dopaininergic, cholinergic, adenosinergic, and a.2-noradrenergic) receptors, glutarnatergic, TRPV1 receptors, PKC and' K+ channels systems, but not L.-a-rg/1\10/cGMP pathway. (C) 2016 SOciedade Brasileira de Farrnacognosia. Published by Elsevier Editora Ltda.
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