Rhamnolipid production from waste cooking oil using Pseudomonas SWP-4

• Main Authors: Lan, Guihong (Author), Fan, Qiang (Author), Liu, Yongqiang (Author), Chen, Chao (Author), Li, Guixiang (Author), Liu, Yu (Author), Yin, Xiaobo (Author)
• Format: Article
• Language: English
• Published: 2015-09-15.
• Subjects: Article
• Online Access: Get fulltext

 The present work aims to produce rhamnolipid from waste cooking oil (WCO) using a newly isolated bacterium named Pseudomonas SWP-4. SWP-4 was a high-yield strain that could accumulate rhamnolipid steadily even in decline phase and gave a maximum rhamnolipid yield of 13.93 g/L and WCO utilization percent around 88%. The critical micelle concentration of the produced rhamnolipid was only 27 mg/L and its emulsification index against n-hexadecane reached around 59%. Moreover, it reduced the surface tension of water from 71.8 mN/m to 24.1 mN/m and the interfacial tension against n-hexadecane from 29.4 mN/m to 0.9 mN/m. Results of biosurfactant stability show the rhamnolipid was effective when the salinity was lower than 8% and pH value ranged from 4 to 10, and it was quite thermostable based on thermal gravity analysis. Furthermore, it maintained high surface activity even after incubation under extreme conditions i.e. pH of 4.0, salinity of 8% and temperature of 80°C for half a month. Based on free fatty acids metabolism analysis, Pseudomonas SWP-4 consumed palmitic acid, oleic acid and linoleic acid chiefly. All these characteristics demonstrate bioconversion and biodegradation of WCO by Pseudomonas SWP-4 is a promising and commercial way of rhamnolipid production and waste treatment.