Site distribution in resin beads as determined by confocal Raman spectroscopy

Site distribution in resin beads as determined by confocal Raman spectroscopy

Scanning confocal Raman spectroscopy was used to study the distribution of reactive sites within a resin bead used for solid-phase synthesis. The distribution of NH2 groups in aminomethylated polystyrene resin (APS) was determined by doping with varying amounts of 4-cyanobenzoic acid. The extent of...

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Bibliographic Details
Main Authors: Kress, Jürgen (Author), Rose, Abigail (Author), Frey, Jeremy G. (Author), Brocklesby, William S. (Author), Ladlow, Mark (Author), Mellor, Geoff W. (Author), Bradley, Mark (Author)
Format: Article
Language:English
Published: 2001-09-17.
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Summary:Scanning confocal Raman spectroscopy was used to study the distribution of reactive sites within a resin bead used for solid-phase synthesis. The distribution of NH2 groups in aminomethylated polystyrene resin (APS) was determined by doping with varying amounts of 4-cyanobenzoic acid. The extent of loading was determined by both elemental analysis and ninhydrin assays. The spatial distribution of the coupled 4-cyanobenzamide within the bead was determined to an in-plane resolution of 1µm and depth resolution of about 4µm, using; the strong Raman CN stretching vibrational transition at 2230 cm(-1). Dry and swollen beads were studied and the distribution was found to be essentially uniform throughout the bead in all cases.

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