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02222nam a2200421Ia 4500 |
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10.3390-s22093252 |
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220510s2022 CNT 000 0 und d |
| 020 |
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|a 14248220 (ISSN)
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|a Fluorescence DNA Switch for Highly Sensitive Detection of miRNA Amplified by Duplex-Specific Nuclease
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| 260 |
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|b MDPI
|c 2022
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| 856 |
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|z View Fulltext in Publisher
|u https://doi.org/10.3390/s22093252
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|a DNA is a type of promising material for the construction of sensors owing to its sequence programmability to control the formation of certain structures. MicroRNA (miRNA) can be applied as promising biomarkers for the diagnosis of a range of diseases. Herein, a novel fluorescent sensing strategy for miRNA is proposed combining duplex-specific nuclease (DSN)-mediated amplification and dumbbell DNA structural switch. Gold nanoparticles (AuNPs) are employed, which provide a 3D reaction interface. They also act as effective fluorescence quenchers. The proposed sensor exhibits high sensitivity (sub-femtomolar level) with a wide dynamic range. In addition, excellent selectivity to distinguish homology sequences is achieved. It also performs satisfactorily in biological samples. Overall, this fluorescent sensor provides a powerful tool for the analysis of miRNA levels and can be applied for related biological studies and clinical diagnosis. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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|a Diagnosis
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|a DNA
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|a DNA switch
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|a duplex-specific nuclease
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|a Duplex-specific nuclease
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|a Dynamic range
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|a Fiber optic sensors
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|a Fluorescence
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|a Fluorescence quenchers
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|a Fluorescent sensing
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|a fluorescent sensor
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|a Fluorescent sensors
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|a gold nanoparticles
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|a Gold nanoparticles
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|a High sensitivity
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|a Highly sensitive detections
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|a Its sequences
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|a Metal nanoparticles
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|a miRNA
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| 650 |
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|a Programmability
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| 650 |
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|a RNA
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|a Guo, Z.
|e author
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|a Li, X.
|e author
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1 |
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|a Luo, G.
|e author
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1 |
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|a Miao, P.
|e author
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| 773 |
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|t Sensors
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