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| LEADER |
01907nam a2200217Ia 4500 |
| 001 |
10.3390-ijms23147497 |
| 008 |
220718s2022 CNT 000 0 und d |
| 020 |
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|a 16616596 (ISSN)
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| 245 |
1 |
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|a Potential of Single Pulse and Multiplexed Drift-Tube Ion Mobility Spectrometry Coupled to Micropillar Array Column for Proteomics Studies
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| 260 |
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|b MDPI
|c 2022
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| 856 |
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|z View Fulltext in Publisher
|u https://doi.org/10.3390/ijms23147497
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| 520 |
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|a Proteomics is one of the most significant methodologies to better understand the molecular pathways involved in diseases and to improve their diagnosis, treatment and follow-up. The investigation of the proteome of complex organisms is challenging from an analytical point of view, because of the large number of proteins present in a wide range of concentrations. In this study, nanofluidic chromatography, using a micropillar array column, was coupled to drift-tube ion mobility and time-of-flight mass spectrometry to identify as many proteins as possible in a protein digest standard of HeLa cells. Several chromatographic parameters were optimized. The high interest of drift-tube ion mobility to increase the number of identifications and to separate isobaric coeluting peptides was demonstrated. Multiplexed drift-tube ion mobility spectrometry was also investigated, to increase the sensitivity in proteomics studies. This innovative proteomics platform will be useful for analyzing patient samples to better understand unresolved disorders. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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|a drift-tube ion mobility spectrometry
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| 650 |
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|a micropillar array column
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| 650 |
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|a multiplexing
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| 650 |
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4 |
|a proteomics
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| 700 |
1 |
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|a Cobraiville, G.
|e author
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|a Fillet, M.
|e author
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|a Gou, M.-J.
|e author
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|a Nix, C.
|e author
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| 773 |
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|t International Journal of Molecular Sciences
|x 16616596 (ISSN)
|g 23 14
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