Light Sheet Fluorescence Microscopy Illuminating Soft Matter

Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has seen spectacular growth in the biological sci...

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Bibliographic Details
Main Authors: McGorty, R. (Author), You, R. (Author)
Format: Article
Language:English
Published: Frontiers Media S.A. 2021
Subjects:
Online Access:View Fulltext in Publisher
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020 |a 2296424X (ISSN) 
245 1 0 |a Light Sheet Fluorescence Microscopy Illuminating Soft Matter 
260 0 |b Frontiers Media S.A.  |c 2021 
856 |z View Fulltext in Publisher  |u https://doi.org/10.3389/fphy.2021.760834 
520 3 |a Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has seen spectacular growth in the biological sciences over the past two decades. In this perspective, we highlight how LSFM may also apply to the field of soft matter. We review the principles and recent advances of LSFM and discuss how it has been used in prior soft matter studies. We demonstrate how a recent implementation of LSFM can be used to study capillary wave fluctuations and droplet coalescence in a colloidal fluid system. Copyright © 2021 You and McGorty. 
650 0 4 |a 3D imaging 
650 0 4 |a capillary waves 
650 0 4 |a colloidal fluids 
650 0 4 |a light sheet fluorescence microscopy 
650 0 4 |a microscopy 
700 1 |a McGorty, R.  |e author 
700 1 |a You, R.  |e author 
773 |t Frontiers in Physics