Isolation and characterisation of gibberellin 2-oxidase 6 gene from oil palm (Elaeis guineensis Jacq.)

• Main Authors: Adura, Z.S.M.S (Author), Nurniwalis, A.W (Author), Zubaidah, R. (Author)
• Format: Article
• Language: English
• Published: Lembaga Minyak Sawit Malaysia 2021
• Subjects: Expression; GA 2-oxidase; Isolation; Mesocarp; Oil palm
• Online Access: View Fulltext in Publisher

 To increase our understanding on the role of Gibberellin (GA) metabolism in oil palm (Elaeis guineensis), a gene encoding enzyme in GA catabolic pathways was identified and isolated. The oligonucleotide primers were constructed and used to amplify the EgGA2ox6 gene from oil palm. The sequence analysis of EgGA2ox6 gene provided a complete open reading frame (ORF) of 1014 bp which encodes 337 deduced amino acid residues. Homology sequence analysis has shown a 50%-70% sequence similarity to GA from other plant species. Based on this sequence motif analysis, the EgGA2ox6 is classified into C19-GA2ox group. Expression studies on 13 different oil palm tissues showed that the highest expression profile of the gene was observed in the mesocarp tissue. Further expression analysis was conducted on the development stages of mesocarp using quantitative real-time polymerase chain reaction (qRT-PCR) showed that the gene was predominantly expressed in the early stages of mesocarp development at four weeks after anthesis (WAA). Then, the expression of EgGA2ox6 gene was gradually reduced as the fruit ripened. This preliminary study provides valuable resources to further elucidate the roles of GA2ox6 gene during oil palm fruit development. © 2021 Lembaga Minyak Sawit Malaysia. All rights reserved.