Microbial load after selective and complete caries removal in permanent molars: A randomized clinical trial

• Main Authors: Bitello-Firmino, L. (Author), Damé-Teixeira, N. (Author), Maltz, M. (Author), Parolo, C.C.F (Author), Soares, V.K (Author)
• Format: Article
• Language: English
• Published: Associacao Brasileira de Divulgacao Cientifica 2018
• Subjects: adolescent; Adolescent; adult; Adult; anaerobic bacterium; Bacteria, Anaerobic; bacterial load; Bacterial Load; case control study; Case-Control Studies; child; Child; controlled study; dental caries; Dental Caries; Dentinal caries; diagnostic imaging; double blind procedure; Double-Blind Method; female; Female; fissure sealant; human; Humans; isolation and purification; Lactobacillus; male; Male; microbiology; Microbiology; Molar; molar tooth; Permanent molars; Pit and Fissure Sealants; Randomized clinical trial; randomized controlled trial; Selective caries removal; Streptococcus; young adult; Young Adult
• Online Access: View Fulltext in Publisher

 The aim of this randomized clinical trial was to compare the remaining microbial load after treatments based on complete and selective caries removal and sealing. Patients with active carious lesions in a permanent molar were randomly allocated into 2 groups: a test group (selective caries removal–SCR; n=18) and a control group (complete caries removal – CCR; n=18). Dentin samples were collected following the excavation and three months after sealing. Streptococcus species, Streptococcus mutans, Lactobacillus species, and total viable microorganisms were cultured to count the viable cells and frequency of species isolation. CCR resulted in significant lower total viable microorganisms counts (p≤0.001), Streptococcus species (p≤0.001) and Lactobacillus species (p≤0.001) initially. However, after sealing, a decrease in total viable microorganisms, Streptococcus species, and Lactobacillus species in the SCR resulted in no difference between the groups after 3 months. In conclusion, selective caries removal is as effective as complete caries removal in reducing dentin bacterial load 3 months after sealing. © 2018, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.