Optimization of photo-biomodulation therapy for wound healing of diabetic foot ulcers in vitro and in vivo

• Main Authors: Che, B. (Author), Chen, Q. (Author), Gu, Y. (Author), Li, H. (Author), Li, Y. (Author), Qiu, H. (Author), Xiafei, S. (Author), Xiaoxi, D. (Author), Yang, H. (Author), Yang, J. (Author), Yin, H. (Author)
• Format: Article
• Language: English
• Published: Optica Publishing Group (formerly OSA) 2022
• Subjects: Bio-modulation; Cell proliferation; Diabetic foot ulcer; Diabetic wounds; Diseases; In-vitro; Optical parameter; Optimisations; Rat model; Vitro and in vivo; Wound cells; Wound healing
• Online Access: View Fulltext in Publisher
• Physical Description: 17
• ISBN: 21567085 (ISSN)
• DOI: 10.1364/BOE.451135

Unclear optical parameters make photo-biomodulation (PBM) difficult to implement in diabetic foot ulcer (DFU) clinically. Here, 12 wavelengths (400-900 nm) were used to conduct PBM to heal DFU wounds in vitro and in vivo. PBM at 10 mW/cm2 and 0.5-4 J/cm2 with all 12 wavelengths promoted proliferation of diabetic wound cells. In a mimic DFU (mDFU) rat model, PBM (425, 630, 730, and 850 nm, and a combination light strategy) promoted mDFU healing. The positive cell proliferation, re-epithelialization, angiogenesis, collagen synthesis, and inflammation were possible mechanisms. The combination strategy had the best effect, which can be applied clinically. © 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement