Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway
Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator use...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society
2023
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| Subjects: | |
| Online Access: | View Fulltext in Publisher View in Scopus |
| LEADER | 01888nam a2200289Ia 4500 | ||
|---|---|---|---|
| 001 | 10.1021-acsphyschemau.3c00003 | ||
| 008 | 230529s2023 CNT 000 0 und d | ||
| 020 | |a 26942445 (ISSN) | ||
| 245 | 1 | 0 | |a Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway |
| 260 | 0 | |b American Chemical Society |c 2023 | |
| 856 | |z View Fulltext in Publisher |u https://doi.org/10.1021/acsphyschemau.3c00003 | ||
| 856 | |z View in Scopus |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-85159726395&doi=10.1021%2facsphyschemau.3c00003&partnerID=40&md5=d53c217e51199bcd8c9b48c305d9441a | ||
| 520 | 3 | |a Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a GEVI subclass known for their high voltage sensitivity and fast response kinetics. Here we review the essential aspects of microbial rhodopsin photocycles that are critical to understanding the mechanisms of voltage sensitivity in these proteins and link them to insights from efforts to create faster, brighter and more sensitive microbial rhodopsin-based GEVIs. © 2023 The Authors. Published by American Chemical Society. | |
| 650 | 0 | 4 | |a biosensors |
| 650 | 0 | 4 | |a fluorescence microscopy |
| 650 | 0 | 4 | |a genetically encoded voltage indicators |
| 650 | 0 | 4 | |a microbial rhodopsins |
| 650 | 0 | 4 | |a neuroscience |
| 650 | 0 | 4 | |a photophysics |
| 650 | 0 | 4 | |a protein engineering |
| 650 | 0 | 4 | |a proton transfer pathway |
| 700 | 1 | 0 | |a Brinks, D. |e author |
| 700 | 1 | 0 | |a Ganapathy, S. |e author |
| 700 | 1 | 0 | |a Meng, X. |e author |
| 700 | 1 | 0 | |a Post, M. |e author |
| 700 | 1 | 0 | |a van Roemburg, L. |e author |
| 773 | |t ACS Physical Chemistry Au | ||