Haemostatic parameters, platelet activation markers, and platelet indices among regular plateletpheresis donors

• Main Authors: Abdullah, W.Z (Author), Hock, L.S (Author), Tuan, H.T.M (Author)
• Format: Article
• Language: English
• Published: Elsevier 2018
• Subjects: anticoagulant protein; antithrombin; Article; comparative study; controlled study; donor; dual specificity phosphatase 2; Haemostatic parameters; hemostasis; human; human experiment; mean platelet volume; PADGEM protein; partial thromboplastin time; Platelet activation markers; platelet count; platelet distribution width; Platelet indices; Plateletpheresis; protein C; protein S; prothrombin time; thrombocyte activation; thrombocytopheresis; von Willebrand factor
• Online Access: View Fulltext in Publisher; View in Scopus
• ISBN: 16583612 (ISSN)
• DOI: 10.1016/j.jtumed.2017.10.006

Objective: Plateletpheresis is generally a safe procedure for platelet donation. Studies on the effects of haemostatic parameters and possible association between automated plateletpheresis and hypercoagulable state are limited. Hence, this study aimed to investigate the effects of plateletpheresis on regular donors using haemostatic parameters, i.e. natural anticoagulant proteins, platelet indices, and platelet activation markers. Methods: A total of 139 participants (plateletpheresis donors and normal controls) were recruited and divided into two groups: Group 1 participants who underwent tests for haemostatic and platelet indices and Group 2 participants who underwent tests for platelet activation markers using CD62P and PAC-1 monoclonal antibodies. Results: A significant mild shortening of prothrombin time and platelet activation were demonstrated (by increased CD62P and PAC-1 markers) among regular plateletpheresis donors as compared to healthy controls. The current pre-donation platelet count of plateletpheresis donors was significantly lower than their mean baseline platelet count obtained before their first plateletpheresis procedure. However, no significant differences were observed for the other platelet parameters (platelet count, mean platelet volume, platelet distribution width, activated partial thromboplastin time, protein C, protein S, antithrombin, and von Willebrand Factor antigen) between plateletpheresis donors and healthy controls. Conclusion: This study concludes that regular plateletpheresis is a safe procedure. A possibility of mild platelet activation among regular donors requires further confirmation. However, pre-analytical platelet and FVII activations could occur in vitro contributing to these findings. © 2017 The Authors