Enamel-like tissue regeneration by using biomimetic enamel matrix proteins

• Main Authors: Cao, C.Y (Author), Fang, Z. (Author), Guo, M. (Author), Li, Q. (Author), Wong, H.M (Author), Zhou, Q. (Author)
• Format: Article
• Language: English
• Published: Elsevier B.V. 2021
• Subjects: amelogenesis; Amelogenesis; amelogenin; Article; biomimetic material; Biomimetic Materials; Biomimetic regeneration; biomimetics; cell culture; cell proliferation; Cell Proliferation; Cells, Cultured; chemical structure; chemistry; controlled study; crystal structure; crystallization; Crystallization; Dental Enamel; Dental Enamel Proteins; enamel; Enamel; enamel matrix proteins; enamel protein; Extracellular matrix proteins; Fourier transform infrared spectroscopy; hierarchical microstructure; human; human tissue; hydroxyapatite; leucine-rich amelogenin peptide; mechanics; mesenchymal stem cell; Mesenchymal Stem Cells; metabolism; microenvironment; modified leucine rich amelogenin peptide; molecular docking; Molecular Docking Simulation; molecular dynamics; Molecular Dynamics Simulation; molecular interaction; protein conformation; Protein Conformation; regeneration; Regeneration; scleroprotein; static electricity; structure activity relation; Structure-Activity Relationship; tissue engineering; Tissue Engineering; tissue regeneration; ultrastructure; unclassified drug; Van der Walls; X ray diffraction
• Online Access: View Fulltext in Publisher

 Enamel regeneration currently -is limited by our inability to duplicate artificially its complicated and well-aligned hydroxyapatite structure. The initial formation of enamel occurs in enamel organs where the ameloblasts secret enamel extracellular matrix formed a unique gel-like microenvironment. The enamel extracellular matrix is mainly composed by amelogenin and non-amelogenin. In this study, an innovative strategy was proposed to regenerate enamel-like tissue by constructing a microenvironment using biomimetic enamel matrix proteins (biomimetic EMPs) composed of modified leucine-rich amelogenin peptide (mLRAP) and non-amelogenin analog (NAA). Impressively, the regenerated enamel in this biomimetic EMPs on etched enamel surface produced prismatic structures, and showed similar mechanical properties to natural enamel. The results of X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) showed that regenerated crystal was hydroxyapatite. Molecular dynamics simulation analysis showed the binding energy between mLRAP and NAA were electrostatic forces and Van der Walls. These results introduced a promising strategy to induce crystal growth of enamel-like hydroxyapatite for biomimetic reproduction of materials with complicated hierarchical microstructures. © 2021 The Authors