Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells

While exposure of C17.2 neural progenitor cells (NPCs) to nanomolar concentrations of carbon nanotubes (NTs) yields evidence of cellular substructure reorganization and alteration of cell division and differentiation, the mechanisms of NT entry are not understood. This study examines the entry modes...

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Bibliographic Details
Main Authors: Chandrasekar, S. (Author), Ignatova, T. (Author), Jedlicka, S.S (Author), Kuipa, S. (Author), Rotkin, S.V (Author), Vargas, A.I (Author)
Format: Article
Language:English
Published: Biophysical Society 2022
Online Access:View Fulltext in Publisher
LEADER 02191nam a2200193Ia 4500
001 10.1016-j.bpr.2022.100061
008 220718s2022 CNT 000 0 und d
020 |a 26670747 (ISSN) 
245 1 0 |a Cell cycle-dependent endocytosis of DNA-wrapped single-walled carbon nanotubes by neural progenitor cells 
260 0 |b Biophysical Society  |c 2022 
856 |z View Fulltext in Publisher  |u https://doi.org/10.1016/j.bpr.2022.100061 
520 3 |a While exposure of C17.2 neural progenitor cells (NPCs) to nanomolar concentrations of carbon nanotubes (NTs) yields evidence of cellular substructure reorganization and alteration of cell division and differentiation, the mechanisms of NT entry are not understood. This study examines the entry modes of (GT)20 DNA-wrapped single-walled carbon nanotubes (SWCNTs) into NPCs. Several endocytic mechanisms were examined for responsibility in nanomaterial uptake and connections to alterations in cell development via cell-cycle regulation. Chemical cell-cycle arrest agents were used to synchronize NPCs in early G1, late G1/S, and G2/M phases at rates (>80%) aligned with previously documented levels of synchrony for stem cells. Synchronization led to the highest reduction in SWCNT internalization during the G1/S transition of the cell cycle. Concurrently, known inhibitors of endocytosis were used to gain control over established endocytic machineries (receptor-mediated endocytosis (RME), macropinocytosis (MP), and clathrin-independent endocytosis (CIE)), which resulted in a decrease in uptake of SWCNTs across the board in comparison with the control. The outcome implicated RME as the primary mechanism of uptake while suggesting that other endocytic mechanisms, though still fractionally responsible, are not central to SWCNT uptake and can be supplemented by RME when compromised. Thereby, endocytosis of nanomaterials was shown to have a dependency on cell-cycle progression in NPCs. © 2022 The Authors 
700 1 |a Chandrasekar, S.  |e author 
700 1 |a Ignatova, T.  |e author 
700 1 |a Jedlicka, S.S.  |e author 
700 1 |a Kuipa, S.  |e author 
700 1 |a Rotkin, S.V.  |e author 
700 1 |a Vargas, A.I.  |e author 
773 |t Biophysical Reports