MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FOXO1 protein complex

• Main Authors: Chen, J. (Author), Chen, L. (Author), Li, X. (Author), Lin, W. (Author), Wei, W. (Author), Wen, X. (Author), Zhang, L. (Author)
• Format: Article
• Language: English
• Published: Elsevier B.V. 2022
• Subjects: Adipogenesis; AdipoQ; C/EBPα; FOXO1; miR-144
• Online Access: View Fulltext in Publisher

 CeRNA effect was an important regulation mode of miRNA mediated bio-activities, however, most of the researches of ceRNA were on ncRNAs synergetic with mRNAs, the exploration of ceRNA effect regulated mRNA interaction was still lack of. Besides, C/EBPα was one of the most crucial adipogenic regulators, which has been demonstrated to form a protein complex with FOXO1 to mediate AdipoQ expression. So that, we try to explore whether the ceRNA effect mediated the interaction of C/EBPα and FOXO1, and identified the key miRNAs of their ceRNA effect. In this paper, we found the ceRNA effect of C/EBPα and FOXO1 mediated their protein complex formation, furthermore regulated its transcriptional role for AdipoQ, thereby influencing pre-adipocytes adipogenesis. More importantly, we demonstrated that the miR-144 was the decisive factor that mediated the ceRNA effect of C/EBPα and FOXO1 to influence AdipoQ, thus regulated pre-adipocytes adipogenesis. This research will provide a new supplementary idea of the miRNA role in mediating coding RNA interaction that regulates pre-adipocyte adipogenesis. © 2022 Elsevier Inc.