Cell surface display system for Lactococcus lactis: A novel development for oral vaccine

Cell surface display system for Lactococcus lactis: A novel development for oral vaccine

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The food-grade Lactococcus lactis is a potential vector to be used as a live vehicle for the delivery of heterologous proteins for vaccine and pharmaceutical purposes. We constructed a plasmid vector pSVac that harbors a 255-bp single-repeat sequence of the cell wall-binding protein region of the Ac...

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Bibliographic Details
Main Authors: Foo, H.L (Author), Raha, A.R (Author), Ross, E. (Author), Varma, N.R.S (Author), Yusoff, K. (Author)
Format: Article
Language:English
Subjects:
amino acid sequence
article
Assays
autolysin
Bacillus sphaericus
Bacteria
bacterial cell
bacterial enzyme
bacterial genetics
bacterial strain
bacterium culture
binding affinity
binding site
Blot analysis
cell surface
cell wall
Cell Wall
Cells
drug carrier
Enterovirus 71
enzyme linked immunosorbent assay
epitope
Epitopes
Escherichia coli
gene construct
gene expression
Gene Expression
Gene fragments
Genes
Gram positive bacterium
Human enterovirus 71
hybrid protein
immunofluorescence
Immunofluorescence
Lactobacillus
Lactobacillus delbrueckii
Lactobacillus vaccine
Lactococcus
Lactococcus lactis
Membrane Proteins
microbiology
molecular cloning
Muramidase
n acetylmuraminidase
nonhuman
Organisms, Genetically Modified
plasmid vector
Plasmids
polyacrylamide gel electrophoresis
Posibacteria
promoter region
protein binding
Protein Binding
protein domain
protein expression
protein isolation
protein purification
protein stability
protein VP1
Proteins
Recombinant Fusion Proteins
recombinant plasmid
recombinant protein
surface property
unclassified drug
Vaccines
Vaccines, Synthetic
Western blotting
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