Reduced expression of circRNA hsa_circ_001888 in gastric cancer and its clinical significance

• Main Authors: Ding, X. (Author), Shi, P. (Author), Song, H. (Author)
• Format: Article
• Language: English
• Published: John Wiley and Sons Inc 2021
• Subjects: adult; aged; AGS cell line; apoptosis; Apoptosis; Article; biomarker; Biomarkers, Tumor; cancer diagnosis; cancer patient; cancer tissue; carcinoembryonic antigen; carcinogenesis; cell proliferation; Cell Proliferation; China; circular ribonucleic acid; circular RNA; clinical feature; controlled study; diagnosis; diagnostic test accuracy study; diagnostic value; disease marker; down regulation; female; Female; gastric cancer; gene expression level; gene expression regulation; Gene Expression Regulation, Neoplastic; gene function; genetic association; genetics; histopathology; hsa circ 001888; hsa_circ_001888; human; human cell; human tissue; Humans; major clinical study; male; Male; metabolism; middle aged; Middle Aged; MKN45 cell line; pathology; plasma; prognosis; Prognosis; protein blood level; receiver operating characteristic; reverse transcription polymerase chain reaction; RNA, Circular; ROC Curve; sensitivity and specificity; stomach cancer; Stomach Neoplasms; stomach tumor; tissue differentiation; tumor cell culture; Tumor Cells, Cultured; tumor marker; unclassified drug
• Online Access: View Fulltext in Publisher

 Background: Circular RNAs (circRNAs) are a novel family of endogenous RNAs. Recent studies have demonstrated that circRNAs are potential novel biomarkers for diagnosing cancers. However, little is known about the role of circRNAs in gastric cancer (GC). This study aimed to identify the relationship between GC and a new circRNA named hsa_circ_001888. Methods: Hsa_circ_001888 expression levels were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in GC cell lines, tissues, and plasma samples. Then, the associations between the expression level of hsa_circ_001888 and the clinicopathological features of patients with GC were further investigated. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value of hsa_circ_001888. Results: In this study, hsa_circ_001888 was first found to be significantly downregulated in GC cell lines (AGS and MKN-45), tissues, and plasma samples compared to control samples. Clinicopathological features showed that the expression of hsa_circ_001888 in GC tissues was associated with differentiation and in GC plasma linked with serum CEA and CA19-9 levels. The areas under the ROC curves of hsa_circ_001888 in tissues and plasma were 0.654 and 0.66, respectively. Conclusions: Hsa_circ_001888 may serve as a potential biomarker in the diagnosis of GC and may be involved in GC development. © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.