Effects of m6A RNA methylation regulators on endometrial cancer

• Main Authors: Yang, Y. (Author), Zhang, Y. (Author)
• Format: Article
• Language: English
• Published: John Wiley and Sons Inc 2021
• Subjects: 6 n methyladenine; 6-methyladenine; adenine; Adenine; ALKBH5 gene; alkylated DNA repair protein alkB homolog 5; alpha ketoglutarate dependent dioxygenase FTO; Alpha-Ketoglutarate-Dependent Dioxygenase FTO; Article; bioinformatic analysis; Biomarkers, Tumor; cancer prognosis; cancer risk; carcinogenesis; case control study; Case-Control Studies; chemistry; clinical feature; controlled study; down regulation; endocytosis; endometrial cancer; Endometrial Neoplasms; endometrium cancer; endometrium tumor; epidermal growth factor receptor; female; Female; FMR1 gene; follow up; Follow-Up Studies; fragile X mental retardation protein; FTO gene; FTO protein, human; gene; gene expression; gene expression regulation; Gene Expression Regulation, Neoplastic; gene ontology; gene set enrichment analysis; genetic association; genetics; HDRNPA2B1 gene; high risk patient; HNRNPA2B1 gene; homologous recombination; human; human tissue; Humans; IGF2BP1 gene; IGF2BP2 gene; IGF2BP3 gene; KIAA1429 gene; low risk patient; LRPPRC gene; major clinical study; messenger RNA; methylation; Methylation; METTL13 gene; METTL14 gene; METTL16 gene; overall survival; oxidative phosphorylation; pathology; prognosis; Prognosis; protein degradation; RBM15B gene; RBMX gene; RNA; RNA metabolism; RNA methylation; RNA sequence; RNA stability; somatomedin binding protein 1; somatomedin binding protein 2; somatomedin binding protein 3; survival rate; Survival Rate; TGF beta signaling; transcriptome; transforming growth factor beta; tumor marker; ubiquitin; Wnt protein; Wnt signaling; YTHDC1 gene; YTHDF1 gene; YTHDF2 gene; YTHDF3 gene; ZC3H13 gene
• Online Access: View Fulltext in Publisher
• ISBN: 08878013 (ISSN)
• DOI: 10.1002/jcla.23942

Background: The modification 6-methyladenine (m6A) is the most common type in RNA methylation. Our study aims to explore the bioinformatic analysis of m6A in endometrial cancer. Methods: The expression of 23 m6A RNA methylation regulators was compared through The Cancer Genome Atlas (TCGA) database among 406 endometrial tissue and 19 normal tissue samples. The Wilcoxon test was applied to compare the relationship between the clinicopathological characteristics and expression. Cox regressions were performed to identify the prognostic factors associated with overall survival. Gene ontology (GO) and Gene Set Enrichment Analysis (GSEA) were performed to evaluate the potential pathways. Results: YTHDF2, HNRNPA2B1, HDRNPA2B1, YTHDF1, FMR1, IGF2BP3, METTL13, RBM15B, IGF2BP1, YTHDF3, YTHDC1, ZC3H13 IGF2BP2, KIAA1429, METTL14, RBMX, FTO, ALKBH5, and METTL16 were significantly abnormally expressed in endometrial cancer tissue samples. Both univariate and multivariate Cox regression analyses indicated that age, grade, and risk score were independent risk factors. High expression of FTO was associated with worse overall survival. Conclusion: M6A RNA methylation regulators play vital role in endometrial cancer. © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.