Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions

• Main Authors: Avtaeva, Y.N (Author), Caprnda, M. (Author), Gabbasov, Z.A (Author), Kruzliak, P. (Author), Melnikov, I.S (Author), Mozos, I. (Author), Okhota, S.D (Author), Prosecky, R. (Author), Rodrigo, L. (Author), Zozulya, N.I (Author)
• Format: Article
• Language: English
• Published: John Wiley and Sons Inc 2021
• Subjects: adult; amino acid sequence; antithrombocytic agent; Article; autoimmune thrombocytopenia; blood flow; Blood Platelets; coating (procedure); controlled study; fibrinogen; Fibrinogen; fibrinogen receptor; fibrinogen-coated surface; flow conditions; glycoprotein Ib receptor; Healthy Volunteers; human; human cell; Humans; kinetics; Kinetics; light intensity; light scattering; measurement; metabolism; middle aged; normal human; platelet adhesion; Platelet Adhesiveness; Platelet Aggregation; Platelet Aggregation Inhibitors; Platelet Glycoprotein GPIb-IX Complex; Platelet Glycoprotein GPIIb-IIIa Complex; receptor; receptor blocking; recording of a scattered laser light signal from a fibrinogen-covered surface; thrombin receptor; thrombocyte; thrombocyte adhesion; thrombocyte aggregation; thrombocyte kinetics; thrombocyte rich plasma; unclassified drug; volunteer; von Willebrand disease; whole blood
• Online Access: View Fulltext in Publisher

 Aim: To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. Methods: We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Testing was performed in platelet-rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. Results: The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 ± 0.08V, n = 7) than in PRP (0.05 ± 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. Conclusion: Our system effectively registers platelet adhesion to a fibrinogen-coated surface under controlled-flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics. © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.