Mn2+-Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels

Mn2+-Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels

This paper provides a guideline for optimizing and utilizing Mn2+ Phos-tag gel technology to separate phosphorylated proteins from their unphosphorylated counterparts. It provides key insights into methods for careful sample preparation and experimental directions for determining the appropriate Pho...

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Bibliographic Details
Main Authors: Ferenczi, M.A (Author), Markandran, K. (Author), Shun, L.M (Author), Xuan, J.V.L.E (Author), Yu, H. (Author)
Format: Article
Language:English
Published: John Wiley and Sons Inc 2021
Subjects:
1,3-bis(bis(pyridin-2-ylmethyl)amino)propan-2-ol
acrylic acid resin
Acrylic Resins
Article
electrophoresis
Electrophoresis, Polyacrylamide Gel
human
Humans
image analysis
immunohistochemistry
manganese
mass spectrometry
metabolism
molecular weight
muscle mass
phosphoprotein
Phosphoproteins
phosphoproteomics
phosphorylation
Phosphorylation
Phos-tag
polyacrylamide
polyacrylamide gel electrophoresis
polyvinylidene fluoride
protein expression
protein phosphorylation
pyridine derivative
Pyridines
skeletal muscle
ubiquitination
western blotting
Western blotting
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