Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation

• Main Authors: Blahnová, V.H (Author), Filová, E. (Author), Muchová, J. (Author), Pavliňáková, V. (Author), Vojtová, L. (Author)
• Format: Article
• Language: English
• Published: MDPI 2022
• Subjects: bioceramics; collagen; osteogenesis
• Online Access: View Fulltext in Publisher

 Collagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on cell interactions. The resulting scaffolds thus differed in grain size, changing from nanoscale to microscopic, and possessed diverse morphological characteristics and resorbability. The materials’ biological action was shown on human bone marrow MSCs. Scaffold morphology was identified by SEM. Using vi-ability test, qPCR, and immunohistochemical staining, we evaluated the biological activity of all of the materials. This study revealed that the most suitable scaffold composition for osteogenesis induction is collagen I foam with calcined hydroxyapatite with a pore size of 360±130 µm and mean particle size of 0.130 µm. The expression of osteogenic markers RunX2 and ColI mRNA was pro-moted, and a strong synthesis of extracellular protein osteocalcin was observed. ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.