Digital Microfluidics-Powered Real-Time Monitoring of Isothermal DNA Amplification of Cancer Biomarker

Digital Microfluidics-Powered Real-Time Monitoring of Isothermal DNA Amplification of Cancer Biomarker

We introduce a digital microfluidics (DMF) platform specifically designed to perform a loop-mediated isothermal amplification (LAMP) of DNA and applied it to a real-time amplification to monitor a cancer biomarker, c-Myc (associated to 40% of all human tumors), using fluorescence microscopy. We demo...

Full description

Bibliographic Details
Main Authors:	Águas, H. (Author), Baptista, P.V (Author), Bettencourt, L. (Author), Coelho, B.J (Author), Fortunato, E. (Author), Igreja, R. (Author), Martins, R. (Author), Veigas, B. (Author)
Format:	Article
Language:	English
Published:	MDPI 2022
Subjects:	cancer biomarker digital microfluidics fluorescence detection loop-mediated isothermal amplification real-time nucleic acid amplification monitoring
Online Access:	View Fulltext in Publisher

Similar Items

A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection
by: Beatriz Jorge Coelho, et al.
Published: (2017-11-01)

Digital Microfluidics for Nucleic Acid Amplification
by: Beatriz Coelho, et al.
Published: (2017-06-01)

Fast Prototyping Microfluidics: Integrating Droplet Digital Lamp for Absolute Quantification of Cancer Biomarkers
by: Beatriz Oliveira, et al.
Published: (2020-03-01)

Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification
by: Michael G. Mauk, et al.
Published: (2015-10-01)

Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
by: Aldrik H. Velders, et al.
Published: (2018-02-01)

Rapid Detection of mecA and femA Genes by Loop-Mediated Isothermal Amplification in a Microfluidic System for Discrimination of Different Staphylococcal Species and Prediction of Methicillin Resistance
by: Xiangrui Meng, et al.
Published: (2020-07-01)

Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
by: Giuseppe Spoto, et al.
Published: (2012-12-01)

A stoichiometric and pseudo kinetic model of loop mediated isothermal amplification
by: Navjot Kaur, et al.
Published: (2020-01-01)

Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method
by: J. Abdullah, et al.
Published: (2014-12-01)

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests
by: Michael G. Mauk, et al.
Published: (2018-02-01)

Loop-Mediated Isothermal Amplification as a Simple Molecular Method for the Detection of Derzsy’s Disease Virus
by: Tarasiuk Karolina, et al.
Published: (2013-03-01)

Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification
by: B. Radhika, et al.
Published: (2016-01-01)

Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens
by: Owen Higgins, et al.
Published: (2018-02-01)

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring
by: Sandra Leonardo, et al.
Published: (2021-01-01)

Rapid detection of Mycoplasma pneumonia by Loop mediated isothermal amplification (LAMP)
by: Davudi-Asl F, et al.
Published: (2015-09-01)

Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA
by: Chuang, H.-S, et al.
Published: (2022)

Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer
by: Yu. A. Makarova, et al.
Published: (2018-07-01)

Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification
by: De-Guo Wang, et al.
Published: (2015-04-01)

Nucleic acid amplification: Alternative methods of polymerase chain reaction
by: Md Fakruddin, et al.
Published: (2013-01-01)

Development of Diagnostic Technology of Xylella fastidiosa Using Loop-Mediated Isothermal Amplification and PCR Methods
by: Suyoung Kim, et al.
Published: (2021-03-01)

Metode Loop-Mediated Isothermal Amplification (LAMP) dan Aplikasinya untuk Deteksi Penyakit Ikan
by: Murwantoko Murwantoko
Published: (2006-02-01)

The Diagnostic Value of a Loop-Mediated Isothermal Amplification (LAMP) Technique for the Detection of Adenovirus Keratitis
by: Mahdokht Mohamadi, et al.
Published: (2013-12-01)

LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) FOR THE DETECTION OF SALMONELLA SPP. ISOLATED FROM DIFFERENT FOOD TYPES
by: Kostas Papanotas, et al.
Published: (2012-08-01)

A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification
by: Pascal Craw, et al.
Published: (2015-09-01)

A Case of Bacillus Calmette-Guérin Cystitis Diagnosed with a Novel Loop-mediated Isothermal Amplification Method
by: Kazuyoshi Tachi, et al.
Published: (2017-09-01)

Simple and visible detection of duck hepatitis B virus in ducks and geese using loop-mediated isothermal amplification
by: Jun Ji, et al.
Published: (2020-02-01)

Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases
by: Pravas Ranjan Sahoo, et al.
Published: (2016-05-01)

Optimization of Loop-Mediated Isothermal Amplification (LAMP) reaction mixture for biosensor applications
by: Sümeyra Vural Kaymaz, et al.
Published: (2021-01-01)

Evaluation of different DNA extraction methods and loop-mediated isothermal amplification primers for the detection of Mycobacterium ulcerans in clinical specimens
by: Anthony Ablordey, et al.
Published: (2021-06-01)

Application of loop-mediated isothermal amplification assay in the detection of herpesvirus of turkey (FC 126 strain) from chicken samples in Nigeria
by: A. J. Adedeji, et al.
Published: (2017-11-01)

Development and Evaluation of Isothermal Amplification Methods for Rapid Detection of Lethal Amanita Species
by: Zhengmi He, et al.
Published: (2019-07-01)

Diagnosis of mycoplasma pneumoniae by loop-mediated isothermal amplification: systematic review and meta-analysis
by: Zi-Hong Cai, et al.
Published: (2019-02-01)

Rapid Detection of Mycoplasma pneumoniae by Loop-Mediated Isothermal Amplification (LAMP) in Clinical Respiratory Specimens
by: Maryam ARFAATABAR, et al.
Published: (2019-05-01)

Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA
by: Na Feng, et al.

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Actinobacillus pleuropneumoniae based the dsbE-like gene
by: Hongwei Ji, et al.
Published: (2012-08-01)

Diagnostic Devices for Isothermal Nucleic Acid Amplification
by: Chia-Chen Chang, et al.
Published: (2012-06-01)

A Smartphone Enabled Molecular Diagnostic Toolkit to Detect Pathogens via Isothermal Nucleic Acid Amplification on Pre-Dried Disposable Paper Strips
by: Masetty, Manaswini
Published: (2021)

Effect of internal primer–template mismatches on loop-mediated isothermal amplification
by: Deguo Wang
Published: (2016-03-01)

Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil
by: Heather M. Kudyba, et al.
Published: (2019-03-01)

Loop-mediated Isothermal Amplification assay for Detection of Candidatus Liberibacter Asiaticus, a Causal Agent of Citrus Huanglongbing
by: Cheol Woo Choi, et al.
Published: (2018-12-01)

Cannot write session to /tmp/vufind_sessions/sess_ihat41p4skr9r5shiqjcbt4mko