Systems biology-based analysis indicates that PHO1;H10 positively modulates high light-induced anthocyanin biosynthesis in Arabidopsis leaves

• Main Authors: Da, L. (Author), Liu, F. (Author), Liu, Y. (Author), Sheng, M. (Author), Song, Q. (Author), Su, Z. (Author), Xu, W. (Author), Zhang, X. (Author)
• Format: Article
• Language: English
• Published: Academic Press Inc. 2022
• Subjects: Anthocyanin biosynthesis; Arabidopsis; Chromatin state; Co-expression network; High light stress responses; PHO1;H10
• Online Access: View Fulltext in Publisher

 Arabidopsis PHO1;H10 is a member of the PHO1 gene family with SPX and EXS domains, and its functions remain largely unknown. As shown in PCSD database, the upstream region of PHO1;H10 gene is in the active chromatin states, with high DHS accessibility and binding sites of multiple transcription factors, especially ABI5, SPCH and HY5. Co-expression network and data-mining analyses showed PHO1;H10 and co-expression genes were with activation under high light stress. We did wet-lab experiments, and found that the detached leaves of PHO1;H10 overexpression lines accumulated more anthocyanin than those of WT and mutant under high light treatment. RNA-seq results showed overexpression of PHO1;H10 up-regulated many anthocyanin biosynthetic genes. The GSEA analysis result showed that the functional module related to anthocyanin pathway was significantly enriched. In summary, we conducted systems biology approach, combining dry- and wet-lab analyses, and discovered that PHO1;H10 might play an essential role during modulating high light-induced anthocyanin accumulation in the Arabidopsis detached leaves. © 2022 The Authors