The Genetics of TCV Resistance

• Main Author: Vaitkunas, Katrina Emilee
• Other Authors: Kristin K. Wobbe, Advisor
• Format: Others
• Published: Digital WPI 2003
• Subjects: Plant resistance; Arabidopsis thaliana; Turnip crinkle virus; Plants; Virus resistance
• Online Access: https://digitalcommons.wpi.edu/etd-theses/428; https://digitalcommons.wpi.edu/cgi/viewcontent.cgi?article=1427&context=etd-theses

Most plants are capable of mounting resistance responses to various pathogen attacks. For a hypersensitive response (HR) to occur, a dominant or semi-dominant resistance (R) plant gene is required to recognize a dominant avirulence (Avr) factor of the pathogen. Three types of Arabidopsis thaliana, Dijon-17 (Di-17), Dijon-3 (Di-3), and Columbia-0 (Col-0), are significant in understanding the genetics of Turnip crinkle virus (TCV) resistance. It has been shown that three genes are needed for successful resistance to TCV in A. thaliana: the dominant R gene HRT, the recessive gene rrt, and a third gene, TIP. Crosses of Di-17 and Di-3 plants, and crosses of Di-3 and Col-0 plants are being analyzed to determine the genotype of the F1 progeny. Using cleaved amplified polymorphic sequence (CAPS) markers, it is possible to determine the genotype of the progeny compared to the wild-type parents at the HRT and TIP loci. Additionally, protein analysis tools will be employed to compare the Di-3 and Di-17 TIP alleles to determine if there are any significant differences in the protein.