Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.

Sex determination, the assignment of sex along a male or female fate, is essential for a species to retain sexual dimorphism and the ability to reproduce sexually. In many organisms, sex-specific transcriptional programs must also be maintained throughout an organism’s lifetime. For instance, if sex...

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Main Author: Rinehart, Leanna
Format: Others
Language:English
Published: W&M ScholarWorks 2017
Subjects:
Online Access:https://scholarworks.wm.edu/etd/1530192330
https://scholarworks.wm.edu/cgi/viewcontent.cgi?article=1262&context=etd
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spelling ndltd-wm.edu-oai-scholarworks.wm.edu-etd-12622021-09-18T05:29:16Z Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila. Rinehart, Leanna Sex determination, the assignment of sex along a male or female fate, is essential for a species to retain sexual dimorphism and the ability to reproduce sexually. In many organisms, sex-specific transcriptional programs must also be maintained throughout an organism’s lifetime. For instance, if sex of stem cells is not continually reinforced, then entire organs could undergo a sex transformation (Zarkower, 2014). Chronologically inappropriate morphogenesis (Chinmo) is a putative transcription factor found in Drosophila that regulates cell fate and behavior. Specifically, it is essential for maintenance of male stem cell sex in the adult testis (Ma et al., 2014). Goals of this research were to investigate how Chinmo is regulated, and how Chinmo controls cell fate and behavior in Drosophila. We hypothesized that key domains and modifiers of Chinmo modulate its function. We found that Chinmo contains several putative SUMO (Small Ubiquitin-Like Modifier) interacting motifs and sumoylation consensus sites that may play a role in regulating its function. Using yeast two-hybrid assays we sought to identify proteins that physically interact with Chinmo, and investigate their functional relevance in the Drosophila testis using RNAi knockdown. This research elucidates the function of the Drosophila protein Chinmo and sheds light on how stem cell sex is properly regulated throughout an organism’s lifetime. 2017-08-03T07:00:00Z text application/pdf https://scholarworks.wm.edu/etd/1530192330 https://scholarworks.wm.edu/cgi/viewcontent.cgi?article=1262&context=etd © The Author http://creativecommons.org/licenses/by-nc/4.0/ Dissertations, Theses, and Masters Projects English W&M ScholarWorks Biology
collection NDLTD
language English
format Others
sources NDLTD
topic Biology
spellingShingle Biology
Rinehart, Leanna
Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.
description Sex determination, the assignment of sex along a male or female fate, is essential for a species to retain sexual dimorphism and the ability to reproduce sexually. In many organisms, sex-specific transcriptional programs must also be maintained throughout an organism’s lifetime. For instance, if sex of stem cells is not continually reinforced, then entire organs could undergo a sex transformation (Zarkower, 2014). Chronologically inappropriate morphogenesis (Chinmo) is a putative transcription factor found in Drosophila that regulates cell fate and behavior. Specifically, it is essential for maintenance of male stem cell sex in the adult testis (Ma et al., 2014). Goals of this research were to investigate how Chinmo is regulated, and how Chinmo controls cell fate and behavior in Drosophila. We hypothesized that key domains and modifiers of Chinmo modulate its function. We found that Chinmo contains several putative SUMO (Small Ubiquitin-Like Modifier) interacting motifs and sumoylation consensus sites that may play a role in regulating its function. Using yeast two-hybrid assays we sought to identify proteins that physically interact with Chinmo, and investigate their functional relevance in the Drosophila testis using RNAi knockdown. This research elucidates the function of the Drosophila protein Chinmo and sheds light on how stem cell sex is properly regulated throughout an organism’s lifetime.
author Rinehart, Leanna
author_facet Rinehart, Leanna
author_sort Rinehart, Leanna
title Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.
title_short Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.
title_full Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.
title_fullStr Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.
title_full_unstemmed Functional Analysis of Chinmo, A Presumed Transcriptional Regulator of Cell Fate and Behavior in Drosophila.
title_sort functional analysis of chinmo, a presumed transcriptional regulator of cell fate and behavior in drosophila.
publisher W&M ScholarWorks
publishDate 2017
url https://scholarworks.wm.edu/etd/1530192330
https://scholarworks.wm.edu/cgi/viewcontent.cgi?article=1262&context=etd
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