Profiling Precursor Lipids for Specialized Pro-Resolution Molecules in Platelet-Rich Plasma Following Fish Oil and Aspirin Intake

• Main Author: Turner, Lisa A
• Format: Others
• Published: VCU Scholars Compass 2017
• Subjects: Platelet-rich plasma; specialized pro-resolution molecules; mass spectrometry; precursor lipids; fish oil; aspirin.; Dentistry; Medicine and Health Sciences; Periodontics and Periodontology
• Online Access: http://scholarscompass.vcu.edu/etd/4763; http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=5854&context=etd

Background: Unfavorable outcomes following periodontal surgeries can be attributed to impaired resolution mechanisms likely due to decreased levels of specialized pro-resolution molecules (SPM). The current study investigates if SPM substrate pools in platelet-rich plasma preparations (PRP) can be increased by essential fatty acid (EFA) and / or aspirin supplementation. Methods: Nineteen healthy volunteers were randomly assigned to take i) aspirin; ii) EFA; iii) aspirin and EFA. Four hours after intake, the lipid precursor pools in PRP were quantified using combined Liquid Chromatography tandem mass spectrometry (LC-MS/MS) and the data statistically analyzed using ANCOVA. Results: Of the 77 metabolites screened, only FFA (18:3) showed a significant interaction effect (p=0.019). By itself, neither EFA (p>0.9) nor aspirin (p>0.4) showed any difference (P>0.4). Multiple comparisons could not identify the differences between groups. Conclusions: There is inadequate data to support oral supplementation of EFA and /or aspirin to increase SPM levels in PRP.