Effects of Nutrient Media on Growth and Morphology of Azotobacter Vinelandii

• Main Author: Butsch, Robert W.
• Other Authors: Vela, G. Roland, 1927-
• Format: Others
• Language: English
• Published: North Texas State University 1974
• Subjects: Azotobacter vinelandii; nutrient media; fungi; Fungi > Cultures and culture media.; Azotobacter.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc935759/

The work described in this thesis was undertaken to study the reasons why Azotobacter vinelandii ATCC 12837 after incubation in Burk's nitrogen-free liquid will not form as many colonies when plated on Difco Tryptic Soy Agar as when planted on Burk's nitrogen-free agar. The difference in growth of A. vinelandii on the two agars was established by performing viable cell-plate counts. The difference in growth was most apparent at 24-hours incubation of the Burk's liquid-media cultures. Phase contrast microscopic observations of Tryptic Soy media cultures of A. vinelandii disclosed the regular formation of fungoid cells at early stages of growth of the bacteria, 18 to 24 hours.