A Rapid, Accurate Thin Layer Chromatographic Analysis of Phospholipids and Neutral Lipids

• Main Author: Wilson, Charlie W.
• Other Authors: Kama, Robert L., 1941-
• Format: Others
• Language: English
• Published: North Texas State University 1979
• Subjects: lipids analysis; thin layer chromatography; health science; Lipids > Analysis.; Thin layer chromatography.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc798059/

A modified ascending thin layer chromatographic technique has been developed which resolves the major phospholipid and neutral lipid classes of five common fluids and tissues. A one-half milliliter sample is extracted with n-butanol:diisopropylether (40:60 by volume, cholesteryl acetate = 100 ng/ul) for thirty minutes and the organic phase is spotted onto a thin layer chromatography plate and carried through three successive chromatographic developments. The lipids are then visualized either by charring with ammonium bisulfate or staining with phosphomolybdic acid. The use of cholesteryl acetate as an internal standard enables quantitation of the phospholipids and neutral lipid classes. This method may be a very valuable, new technique for research and clinical laboratories.