Cigarette Residues Affect Steroidogenesis in Cultured Y-1 Mouse Adrenal Tumor Cells

• Main Author: Morris, Paula D.
• Other Authors: Mrotek, James J.
• Format: Others
• Language: English
• Published: North Texas State University 1979
• Subjects: steroid production; cigarette residues; cyclic AMP; tobacco usage; adrenal tumors; Tobacco > Physiological effect.; Steroid metabolism.; Adrenal glands > Tumors.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc663585/

This study (1) quantitatively compared steroid production in cultured Y-l mouse adrenal tumor cells exposed to Camel and Carlton-smoke derived residues, and (2) localized the effects in the cell. Basal steroid production was increased by Camel residues but not by Carlton, while ACTH stimulation was interfered with by both residues. Camel basal stimulation was comparable to that of cAMP, and was abolished by Cytochalasin D. The stimulation was also comparable to that of cholera toxin, which activates adenyl cyclase. Results indicate that residue components dissolve in the membrane stimulating adenyl cyclase at a point similar to or before that utilized by cholera toxin for its stimulating effect.