Separation of Transition Metal Ions by HPLC, Using UV-VIS Detection

• Main Author: Lien, Wan-Fu
• Other Authors: Tarter, James G.
• Format: Others
• Language: English
• Published: North Texas State University 1987
• Subjects: high performance liquid chromatography; untraviolet-visible detection systems; ion separation; Separation (Technology); Metal ions > Spectra.; Transition metals > Spectra.; High performance liquid chromatography.; Ultraviolet spectrometry.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc501145/

HPLC has been used and can quickly determine several ions simultaneously. The method of determination described for transition metals [Cr(III), Fe(III), Ni(II), Co(II), Cu(II), Zn(II), Cd(II), Mn(II)] and [Ca(II), Pb(II)] using HPLC with UV-VIS detection is better than the PAR complexation method commonly used. The effects of both eluent pH and detector wavelength were investigated. Results from using different pHs and wavelengths, optional analytical conditions for the separation of [Ni(II), Co(II), Cu(II)], [Cr(III), Fe(III), Ca(II), Ni(II), Cu(II)], and [Ca(II), Zn(II), Pb(II)] in one injection, respectively, are described. The influence of adding different concentrations of Na_2EDTA solvent to the sample is shown. Detection limits, linear range, and the comparisons between this study and a post-column PAR method are given.